Herein we show some data on the use of recombinant human TRAIL (rhTRAIL) and gamma-radiation (10 Gy) in combination in an autochthonous mouse model for hepatocellular carcinoma. As cell death signaling through the death receptors is evolutionary conserved from zebra fish to man, novel genetically engineered mouse tumor models may prove useful in establishing in vivo models that excel our fundamental understanding of resistance to TRAIL-death receptor signaling, off-target effects from TRAIL-death receptor

targeting compounds and help in identifying a clinically cogent rationale for efficient targeting of TRAIL death AP26113 Angiogenesis inhibitor receptors in patients. Once established, mouse tumor models may prove to be a useful tool in understanding TRAIL-death receptor signaling.”
“Drosophila ventral furrow formation has frequently been used as a model to study developmentally-regulated cell-shape changes. However, a technique selleck screening library to follow all cellular changes during this process within a single living embryo has been lacking. We describe a novel technique, called “end-on imaging”, to collect time-lapse images of transversely mounted living embryos. End-on imaging revealed several new features of dorsoventral development. First, we observed a wave of syncytial nuclear divisions predicting the location of the ventral furrow. Second, we determined

that there is a 5-min gap between the end of cellularization and the start of ventral furrow formation, suggesting that the two processes JAK inhibitor may share the same pool of cytoskeletal components. Lastly, we show that apical-membrane flattening, the first step in ventral furrow formation, is due to the ventral cells pushing against the vitelline membrane, rather than flattening the dome-shaped, apical surfaces of these cells by a pulling or constriction motion. Developmental Dynamics

237.3252-3259, 2008. (C) 2008 Wiley-Liss, Inc.”
“Under the framework of computer-aided eye disease diagnosis, this paper presents an automatic optic disc (OD) detection technique. The proposed technique makes use of the unique circular brightness structure associated with the OD, i.e., the OD usually has a circular shape and is brighter than the surrounding pixels whose intensity becomes darker gradually with their distances from the OD center. A line operator is designed to capture such circular brightness structure, which evaluates the image brightness variation along multiple line segments of specific orientations that pass through each retinal image pixel. The orientation of the line segment with the minimum/maximum variation has specific pattern that can be used to locate the OD accurately. The proposed technique has been tested over four public datasets that include 130, 89, 40, and 81 images of healthy and pathological retinas, respectively.